Methods in biotechnology

Det helsevitenskapelige fakultet

Spring course.

This course can be taken as a singular course.

BIO-1001 Cell and molecular biology, BIO-2302 Molecular cellbiology, BIO-2303 Genetics, BIO-2304 Introduction to Bioinformatics

The course is particularly aimed at master students of technology and covers the first step in a typical closed biotechnological process to produce a recombinant product. The lab work which forms the greater part of the course, will make use of recombinant DNA technology to clone and express the gene which codes for a known natural protein. The first part of the task will be to construct a gene library (cDNA/genomic). The next step is to identify and characterise the gene that codes for the known protein, and finally the gene will be sub-cloned in an appropriate expression vector that will yield good gene expression in a suitable host. The known natural protein may be from a eukaryotic (e.g. plant, fish, micro-organism) or prokaryotic organism. Bioinformatics will be an important instrument in this work. The lectures will cover relevant topics of recombinant DNA technology / biotechnology and also explain how lab records and reports should be compiled.

The students should master theoretical and technical approaches needed to construct a gene library, to identify and sub-clone a gene for a known protein and attain good gene expression in an appropriate vector and host.

English

Lectures, student colloquia / seminar and compulsory lab work

Submitted lab reports. Grade type: pass / fail.

Re-sit examination
For students who did not pass their exam, or were unable to sit their exam due to medical reasons etc., we offer a re-sit examination early in the following semester.

BIO-352S Methods in biotechnology 9

Syllabus will be available on Classfronter.