Course code: BIO-3137

Applied Molecular Genetics

Campus

Semester / Year Autumn 2013

Credits 10

NOTE! This course belongs to a previous semester/year

About the course

Theoretical and practical subject for masterstudents.

The aim of this course is to give a thorough introduction to molecular genetic methods used in modern biological research. The course will give a basis for advanced studies in different areas of biology and include practical exercises using both environmental and tissue specific samples. The students will isolate DNA and RNA from different samples and use Q-PCR for quantification of specific functional genes and transcripts. The laboratory work is running for two subsequent weeks and the results are discussed in plenum. The students have to write individual laboratory reports. The lectures will be closely connected to the practical problems to be solved in the laboratory. Selected articles from the scientific curriculum are topics for seminar presented by the students.

Objectives of the course

Knowledge: Theory of gene and gene expression analyses Theory of microbial ecology Theory of nucleic acids extraction Critical consideration of scientific literature within the topics for the course How to write a laboratory report Critical assessment of own results Skills: Isolation of DNA and RNA from environmental samples Isolation of RNA from tissue cDNA synthesis and quality control Perform Q-PCR for quantification of gene abundance and expression Planning molecular laboratory work Present objectives, methods and results. Oral and written discussion of results General competence: Theory of environmental genetics Experimental design Molecular laboratory work Evaluation of methods Discussion of results Presentation of results

Prerequisites

Credit reduction

If you pass the examination in this course, you will get an reduction in credits (as stated below), if you previously have passed the following courses:

BIO-3134 Applied Molecular Genetics 10 stp

Teaching methods

Lectures 8 hours, laboratory 40 hours within 2 weeks, seminars 10 hours

Language of instruction and examination

English

Assessment

Laboratory report and oral exam with grades. Letter grades, A-F.

Error rendering component

Previous years and semesters

Kontakt oss

Responsible unit: Institutt for arktisk og marin biologi

Mette Marianne Svenning

Emeriti

mette.svenning@uit.no

Siv Andreassen

Kontorsjef

siv.andreassen@uit.no