Course code: BIO-3318
Methods in biotechnology
Campus
Semester / Year
Autumn 2008
Credits
10
NOTE! This course belongs to a previous semester/year
BIO-3318
Methods in biotechnology
-10 ects
The course is administrated by
Faculty of Medicine
Faculty of Medicine
Type of course
Theoretical and practical.
Theoretical and practical.
Recommended prerequisites
BIO-1001 Cell and molecular biology, BIO-2302 Molecular cellbiology, BIO-2303 Genetics, BIO-2304 Introduction to Bioinformatics
BIO-1001 Cell and molecular biology, BIO-2302 Molecular cellbiology, BIO-2303 Genetics, BIO-2304 Introduction to Bioinformatics
Course contents
The course is particularly aimed at master students of technology and covers the first step in a typical closed biotechnological process to produce a recombinant product. The lab work which forms the greater part of the course, will make use of recombinant DNA technology to clone and express the gene which codes for a known natural protein. The first part of the task will be to construct a gene library (cDNA/genomic). The next step is to identify and characterise the gene that codes for the known protein, and finally the gene will be sub-cloned in an appropriate expression vector that will yield good gene expression in a suitable host. The known natural protein may be from a eukaryotic (e.g. plant, fish, micro-organism) or prokaryotic organism. Bioinformatics will be an important instrument in this work. The lectures will cover relevant topics of recombinant DNA technology / biotechnology and also explain how lab records and reports should be compiled.
The course is particularly aimed at master students of technology and covers the first step in a typical closed biotechnological process to produce a recombinant product. The lab work which forms the greater part of the course, will make use of recombinant DNA technology to clone and express the gene which codes for a known natural protein. The first part of the task will be to construct a gene library (cDNA/genomic). The next step is to identify and characterise the gene that codes for the known protein, and finally the gene will be sub-cloned in an appropriate expression vector that will yield good gene expression in a suitable host. The known natural protein may be from a eukaryotic (e.g. plant, fish, micro-organism) or prokaryotic organism. Bioinformatics will be an important instrument in this work. The lectures will cover relevant topics of recombinant DNA technology / biotechnology and also explain how lab records and reports should be compiled.
Objective of the course
The students should master theoretical and technical approaches needed to construct a gene library, to identify and sub-clone a gene for a known protein and attain good gene expression in an appropriate vector and host.
The students should master theoretical and technical approaches needed to construct a gene library, to identify and sub-clone a gene for a known protein and attain good gene expression in an appropriate vector and host.
Language of instruction and examination
Norwegian or English
Norwegian or English
Teaching methods
Lectures, student colloquia / seminar and compulsory lab work
Lectures, student colloquia / seminar and compulsory lab work
Assessment methods
Submitted lab reports Grade type: pass / fail
Submitted lab reports Grade type: pass / fail
Course overlap
BIO-352S Methods in biotechnology 9
BIO-352S Methods in biotechnology 9
Recommended reading/syllabus
Available on request
Available on request
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