Course code: BIO-3321
Protein purification
Campus
Semester / Year
Autumn 2008
Credits
10
NOTE! This course belongs to a previous semester/year
BIO-3321
Protein purification
-10 ects
The course is administrated by
Faculty of Medicine
Faculty of Medicine
Type of course
Theoretical and practical.
Theoretical and practical.
Recommended prerequisites
BIO-2300 Biochemistry
BIO-2300 Biochemistry
Course contents
The course will cover various subjects: planning purification of protein, choice of source, homogenisation and preparation of extract, chromatographic separation methods, choice of purifying substance and buffers, optimisation of chromatographic separation methods and upgrading purification. Practical work comprises a choice of strategy to purify the protein, optimisation of chromatographic separation methods on a small scale, purification of protein on a large scale and analysis of purified protein.
The course will cover various subjects: planning purification of protein, choice of source, homogenisation and preparation of extract, chromatographic separation methods, choice of purifying substance and buffers, optimisation of chromatographic separation methods and upgrading purification. Practical work comprises a choice of strategy to purify the protein, optimisation of chromatographic separation methods on a small scale, purification of protein on a large scale and analysis of purified protein.
Objective of the course
The student should be able to prepare his own purification strategy and to carry out purification, to present purification charts and to evaluate the results and purity.
The student should be able to prepare his own purification strategy and to carry out purification, to present purification charts and to evaluate the results and purity.
Language of instruction and examination
English
English
Teaching methods
Lectures and lab work; compulsory attendance
Lectures and lab work; compulsory attendance
Assessment methods
Submitted lab report Grade type: pass / fail
Submitted lab report Grade type: pass / fail
Course overlap
BIO-360S Protein purification 9
BIO-360S Protein purification 9
Recommended reading/syllabus
Available on request
Available on request
| Lectures Autumn 2008 Første oppmøte: 18/8 kl. 09.15 på Aud 9, MH-bygget |
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| Forelesning |
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| Laboratorieøvelse 1 |
2008-08-29 2008-08-25 |
| Laboratorieøvelse 2 |
2008-09-05 2008-09-01 |
| Laboratorieøvelse 3 |
2008-09-12 2008-09-08 |
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