Course code: BIO-3318
Methods in biotechnology
Campus
Semester / Year
Spring 2009
Credits
10
NOTE! This course belongs to a previous semester/year
BIO-3318
Methods in biotechnology
-
10
ects
The course is administrated by
Faculty of Medicine
Faculty of Medicine
Type of course
This course can be taken as a singular course.
This course can be taken as a singular course.
Recommended prerequisites
BIO-1001 Cell and molecular biology, BIO-2302 Molecular cellbiology, BIO-2303 Genetics, BIO-2304 Introduction to Bioinformatics
BIO-1001 Cell and molecular biology, BIO-2302 Molecular cellbiology, BIO-2303 Genetics, BIO-2304 Introduction to Bioinformatics
Course contents
The course is particularly aimed at master students of technology and covers the first step in a typical closed biotechnological process to produce a recombinant product. The lab work which forms the greater part of the course, will make use of recombinant DNA technology to clone and express the gene which codes for a known natural protein. The first part of the task will be to construct a gene library (cDNA/genomic). The next step is to identify and characterise the gene that codes for the known protein, and finally the gene will be sub-cloned in an appropriate expression vector that will yield good gene expression in a suitable host. The known natural protein may be from a eukaryotic (e.g. plant, fish, micro-organism) or prokaryotic organism. Bioinformatics will be an important instrument in this work. The lectures will cover relevant topics of recombinant DNA technology / biotechnology and also explain how lab records and reports should be compiled.
The course is particularly aimed at master students of technology and covers the first step in a typical closed biotechnological process to produce a recombinant product. The lab work which forms the greater part of the course, will make use of recombinant DNA technology to clone and express the gene which codes for a known natural protein. The first part of the task will be to construct a gene library (cDNA/genomic). The next step is to identify and characterise the gene that codes for the known protein, and finally the gene will be sub-cloned in an appropriate expression vector that will yield good gene expression in a suitable host. The known natural protein may be from a eukaryotic (e.g. plant, fish, micro-organism) or prokaryotic organism. Bioinformatics will be an important instrument in this work. The lectures will cover relevant topics of recombinant DNA technology / biotechnology and also explain how lab records and reports should be compiled.
Objective of the course
The students should master theoretical and technical approaches needed to construct a gene library, to identify and sub-clone a gene for a known protein and attain good gene expression in an appropriate vector and host.
The students should master theoretical and technical approaches needed to construct a gene library, to identify and sub-clone a gene for a known protein and attain good gene expression in an appropriate vector and host.
Language of instruction and examination
English
English
Teaching methods
Lectures, student colloquia / seminar and compulsory lab work
Lectures, student colloquia / seminar and compulsory lab work
Assessment methods
Submitted lab reports. Grade type: pass / fail.
A re-sit exam will be arranged for this course.
Submitted lab reports. Grade type: pass / fail.
A re-sit exam will be arranged for this course.
Course overlap
BIO-352S Methods in biotechnology 9
BIO-352S Methods in biotechnology 9
Recommended reading/syllabus
Syllabus will be available on Classfronter.
Syllabus will be available on Classfronter.
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Lectures Spring 2009 First attendance: See Schedule. |
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| Forelesning |
The course will be arranged in week 3-9. Since equipment to the lab has to be ordered several weeks before the course begins we urge all students to send an email to student advisor Karin Eilertsen before Wednesday 17th of December, karin.eilertsen@fagmed.uit.no. |
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